Transamidase reactions involved in the enzymic coagulation of semen: isolation of -glutamyl- -lysine dipeptide from clotted secretion protein of guinea pig seminal vesicle.
نویسندگان
چکیده
New supportive evidence is advanced in favor of the hypothesis that the enzymic coagulation of guinea pig semen involves transamidase reactions that result in the formation of gamma-glutamyl-epsilon-lysine intermolecular cross linkages between molecules of a basic protein in seminal vesicle secretion. The dipeptide gamma-glutamyl-epsilon-lysine was isolated in large quantities from proteolytic digests of the coagulated basic vesicular secretion protein that comprises the seminal clot.
منابع مشابه
The major clotting protein from guinea pig seminal vesicle contains eight repeats of a 24-amino acid domain.
The complete amino acid sequence of the major clotting protein from the guinea pig seminal vesicle (SVP-1) has been determined by nucleotide sequencing of cDNA clones corresponding to the 3' terminus of an mRNA that codes for a protein precursor to SVP-1. The first 40 amino acids of the derived protein sequence are identical to those determined by N-terminal sequencing of SVP-1 isolated from th...
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 69 8 شماره
صفحات -
تاریخ انتشار 1972